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pet32a htt ex1q46 plasmid (Addgene inc)

Name: pet32a htt ex1q46 plasmid
Brand: Addgene inc
Rating: 92 (10 reviews)

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Addgene inc pet32a htt ex1q46 plasmid

ERF3 self-assembles in vitro . ( A ) Schematic of the recombinant ERF3 construct. The recombinant ERF3 contains a cleavable Trx tag on the N-terminus to stabilize the protein and a 6xHis tag on the C-terminus to enable nickel purification. ( B and C ) The purified Trx ERF3 protein runs at its expected size (77 kDa). SDS-PAGE gels of the recombinant Trx ERF3 protein were either Coomassie stained ( B ) or analyzed by western blot ( C ). Purified Trx ERF3 protein runs at its expected size of 77 kDa and also ladders at higher molecular weights, indicating potential oligomer formation. ( D ) Removal of the Trx tag results in ERF3 aggregation. Recombinant Trx ERF3 was incubated with empty vehicle or with enterokinase and then analyzed by SDD-AGE and western blot ( n = 5). ( E ) Trx-cleaved ERF3 produces signal by ThT. Recombinant Trx ERF3 or Htt <t>ex1Q46</t> were incubated with enterokinase, and fluorescence of the amyloid-specific dye ThT was monitored ( n = 3; error bars indicate standard deviation). ( F and G ) TEM micrographs of Trx-cleaved recombinant ERF3 and mutant Htt protein. Trx-cleaved ERF3 ( F ) forms large, branched assemblies while Trx-cleaved Htt ( G ) produces highly ordered fibrils.

Pet32a Htt Ex1q46 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Acute stress and multicellular development alter the solubility of the Dictyostelium Sup35 ortholog ERF3"

Article Title: Acute stress and multicellular development alter the solubility of the Dictyostelium Sup35 ortholog ERF3

Journal: Microbiology Spectrum

doi: 10.1128/spectrum.01607-24

Figure Legend Snippet: ERF3 self-assembles in vitro . ( A ) Schematic of the recombinant ERF3 construct. The recombinant ERF3 contains a cleavable Trx tag on the N-terminus to stabilize the protein and a 6xHis tag on the C-terminus to enable nickel purification. ( B and C ) The purified Trx ERF3 protein runs at its expected size (77 kDa). SDS-PAGE gels of the recombinant Trx ERF3 protein were either Coomassie stained ( B ) or analyzed by western blot ( C ). Purified Trx ERF3 protein runs at its expected size of 77 kDa and also ladders at higher molecular weights, indicating potential oligomer formation. ( D ) Removal of the Trx tag results in ERF3 aggregation. Recombinant Trx ERF3 was incubated with empty vehicle or with enterokinase and then analyzed by SDD-AGE and western blot ( n = 5). ( E ) Trx-cleaved ERF3 produces signal by ThT. Recombinant Trx ERF3 or Htt ex1Q46 were incubated with enterokinase, and fluorescence of the amyloid-specific dye ThT was monitored ( n = 3; error bars indicate standard deviation). ( F and G ) TEM micrographs of Trx-cleaved recombinant ERF3 and mutant Htt protein. Trx-cleaved ERF3 ( F ) forms large, branched assemblies while Trx-cleaved Htt ( G ) produces highly ordered fibrils.

Techniques Used: In Vitro, Recombinant, Construct, Purification, SDS Page, Staining, Western Blot, Incubation, Fluorescence, Standard Deviation, Mutagenesis

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Journal: Microbiology Spectrum

Article Title: Acute stress and multicellular development alter the solubility of the Dictyostelium Sup35 ortholog ERF3

doi: 10.1128/spectrum.01607-24

Figure Lengend Snippet: ERF3 self-assembles in vitro . ( A ) Schematic of the recombinant ERF3 construct. The recombinant ERF3 contains a cleavable Trx tag on the N-terminus to stabilize the protein and a 6xHis tag on the C-terminus to enable nickel purification. ( B and C ) The purified Trx ERF3 protein runs at its expected size (77 kDa). SDS-PAGE gels of the recombinant Trx ERF3 protein were either Coomassie stained ( B ) or analyzed by western blot ( C ). Purified Trx ERF3 protein runs at its expected size of 77 kDa and also ladders at higher molecular weights, indicating potential oligomer formation. ( D ) Removal of the Trx tag results in ERF3 aggregation. Recombinant Trx ERF3 was incubated with empty vehicle or with enterokinase and then analyzed by SDD-AGE and western blot ( n = 5). ( E ) Trx-cleaved ERF3 produces signal by ThT. Recombinant Trx ERF3 or Htt ex1Q46 were incubated with enterokinase, and fluorescence of the amyloid-specific dye ThT was monitored ( n = 3; error bars indicate standard deviation). ( F and G ) TEM micrographs of Trx-cleaved recombinant ERF3 and mutant Htt protein. Trx-cleaved ERF3 ( F ) forms large, branched assemblies while Trx-cleaved Htt ( G ) produces highly ordered fibrils.

Article Snippet: Htt ex1 was expressed recombinantly in BL21 cells using the pET32a-Htt ex1Q46 plasmid [Addgene #11515 ( )].

Techniques: In Vitro, Recombinant, Construct, Purification, SDS Page, Staining, Western Blot, Incubation, Fluorescence, Standard Deviation, Mutagenesis

Fig. 3. mCherry expression in cNTS subdivisions labeled via targeted recombination in active populations (TRAP) by VNS in FosCreER mice A. Experimental design for the Fos-TRAP NTS neurons by VNS. Cre-dependent AAV9 encoding mCherry was injected into the cNTS bilaterally (0.1 μl/side). Tamoxifen (75–150 mg/kg) was injected intraperitoneally, and VNS was delivered to TRAP cNTS neurons. B-C. Representative images of mCherry-expressing neurons in the subdivisions of the cNTS after cVNS (B, left), aVNS (C, left), or sham (B and C, right). The location of the brain section is Bregma -7.4 mm. Scale, 100 μm. AP, area postrema; cc, central canal; ST, solitary tract. D. Magnified images of the inset in panel B. Scale, 50 μm.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 3. mCherry expression in cNTS subdivisions labeled via targeted recombination in active populations (TRAP) by VNS in FosCreER mice A. Experimental design for the Fos-TRAP NTS neurons by VNS. Cre-dependent AAV9 encoding mCherry was injected into the cNTS bilaterally (0.1 μl/side). Tamoxifen (75–150 mg/kg) was injected intraperitoneally, and VNS was delivered to TRAP cNTS neurons. B-C. Representative images of mCherry-expressing neurons in the subdivisions of the cNTS after cVNS (B, left), aVNS (C, left), or sham (B and C, right). The location of the brain section is Bregma -7.4 mm. Scale, 100 μm. AP, area postrema; cc, central canal; ST, solitary tract. D. Magnified images of the inset in panel B. Scale, 50 μm.

Article Snippet: Adeno-associated virus (AAV) serotype 9 encoding the Credependent, mCherry reporter protein driven by a human synapsin promoter (AAV-hSyn-DIO-mCherry; Addgene, #50459-AAV9) was bilaterally injected in FosCreER mice into the cNTS (anteroposterior -7.4 mm; lateromedial -1.15 to -1.3 mm; dorsoventral 4.2–4.22 mm) taking stereotaxic coordinate direction from the Mouse Brain Atlas [44].

Techniques: Expressing, Labeling, Injection

Fig. 4. Rostrocaudal distribution of mCherry-positive cNTS neurons TRAPped by cVNS and aVNS A. Representative images showing the rostrocaudal distribution of mCherry+ neurons within the cNTS of mice with cVNS or aVNS. Scale, 100 μm. AP, area postrema; Gr, gracile nucleus; DMV, dorsal motor nucleus of the vagus. B. Total numbers of mCherry+ neurons within the cNTS of mice with cVNS or aVNS in sex-aggregated and disaggregated analyses. cVNS vs aVNS in female, t (5) = 3.91, *p < 0.05 in Student’s t-test. C. Comparisons of the numbers of mCherry+ neurons in the entire cNTS (excluding SolC) in the ipsilateral and contralateral sides in sex-aggregated and disaggregated analyses. (M + F) cVNS ipsi vs contra, t (26) = 5.57, ****p < 0.0001; (M + F) aVNS ipsi vs contra, t (26) = 4.65, ***p < 0.001; (M) cVNS ipsi vs contra, t (12) = 4.72, **p < 0.005; (M) aVNS ipsi vs contra, t (12) = 4.32, ***p < 0.005; (F) cVNS ipsi vs contra, t (10) = 5.65, ***p < 0.001; (F) aVNS ipsi vs contra, t (10) = 4.22, **p < 0.005 in Sidak’s post-test following two-way ANOVA. D. Rostrocaudal distribution of mCherry+ neurons within the cNTS in sex-aggregated analysis. Ten sections from Bregma -7.24 to -7.79 were quantified. N = 7 in the cVNS and 8 in the aVNS. -7.48, t (13) = 8.16, **p < 0.01; -7.55, t (13) = 4.84, **p < 0.01; -7.6,t (13) = 4.18, **p < 0.01; -7.66, t (13) = 3.29, *p < 0.05 in multiple unpaired t-tests using Holm-Sidak’s multiple comparison correction. E-F. Sex-disaggregated analysis. N = 4 in cVNS and 4 aVNS in males (E); 3 in cVNS and 4 aVNS in females (F). (M), -7.48, t (6) = 5.18, *p < 0.05; (F), -7.48, t (5) = 9.27, **p < 0.01; (F) -7.72, t (5) = 5.87, *p < 0.05; (F) -7.76, t (5) = 5.6, *p < 0.05 in multiple unpaired t-tests using Holm-Sidak’s multiple comparison correction.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 4. Rostrocaudal distribution of mCherry-positive cNTS neurons TRAPped by cVNS and aVNS A. Representative images showing the rostrocaudal distribution of mCherry+ neurons within the cNTS of mice with cVNS or aVNS. Scale, 100 μm. AP, area postrema; Gr, gracile nucleus; DMV, dorsal motor nucleus of the vagus. B. Total numbers of mCherry+ neurons within the cNTS of mice with cVNS or aVNS in sex-aggregated and disaggregated analyses. cVNS vs aVNS in female, t (5) = 3.91, *p < 0.05 in Student’s t-test. C. Comparisons of the numbers of mCherry+ neurons in the entire cNTS (excluding SolC) in the ipsilateral and contralateral sides in sex-aggregated and disaggregated analyses. (M + F) cVNS ipsi vs contra, t (26) = 5.57, ****p < 0.0001; (M + F) aVNS ipsi vs contra, t (26) = 4.65, ***p < 0.001; (M) cVNS ipsi vs contra, t (12) = 4.72, **p < 0.005; (M) aVNS ipsi vs contra, t (12) = 4.32, ***p < 0.005; (F) cVNS ipsi vs contra, t (10) = 5.65, ***p < 0.001; (F) aVNS ipsi vs contra, t (10) = 4.22, **p < 0.005 in Sidak’s post-test following two-way ANOVA. D. Rostrocaudal distribution of mCherry+ neurons within the cNTS in sex-aggregated analysis. Ten sections from Bregma -7.24 to -7.79 were quantified. N = 7 in the cVNS and 8 in the aVNS. -7.48, t (13) = 8.16, **p < 0.01; -7.55, t (13) = 4.84, **p < 0.01; -7.6,t (13) = 4.18, **p < 0.01; -7.66, t (13) = 3.29, *p < 0.05 in multiple unpaired t-tests using Holm-Sidak’s multiple comparison correction. E-F. Sex-disaggregated analysis. N = 4 in cVNS and 4 aVNS in males (E); 3 in cVNS and 4 aVNS in females (F). (M), -7.48, t (6) = 5.18, *p < 0.05; (F), -7.48, t (5) = 9.27, **p < 0.01; (F) -7.72, t (5) = 5.87, *p < 0.05; (F) -7.76, t (5) = 5.6, *p < 0.05 in multiple unpaired t-tests using Holm-Sidak’s multiple comparison correction.

Techniques: Comparison

Fig. 5. Distribution of mCherry-positive neurons in different subdivisions of the cNTS following cVNS and aVNS A. Representative images showing subdivisions of the cNTS at Bregma -7.4 after cVNS (upper) or aVNS (lower). Scale, 100 μm. AP, area postrema; cc, central canal; SolDM, solitary nucleus dorsomedial part; SolDL, solitary nucleus dorsolateral part; SolM, solitary nucleus medial part; SolCe, solitary nucleus central part; Psol, parasolitary nucleus; SolVL, solitary nucleus ventrolateral part; ST, solitary tract; DMV, dorsal motor nucleus of the vagus. B. The number of mCherry+ neurons in different subdivisions in the cNTS from -7.24 to -7.79 to Bregma. (M + F) SolDM, t (13) = 17.86, ***p < 0.001; (M + F) SolDL, t (13) = 6.45, ***p < 0.001; (M + F) PSol, t (13) = 8.88, ***p < 0.001; (M + F) SolVL, t (13) = 14.60, ***p < 0.001; (M + F) SolC, t (13) = 3.60, **p < 0.01 in multiple t-tests using the Holm-Sidak multiple comparison correction. C-D. Sex disaggregated analysis. N = 4 in cVNS and 4 aVNS in males (C); 3 in cVNS and 4 aVNS in females (D). (M) SolDM, t (6) = 12.89, ***p < 0.001; (M) SolDL, t (6) = 3.90, *p < 0.05; (M) PSol, t (6) = 5.12, *p < 0.05; (M) SolVL, t (6) = 8.69, ***p < 0.001; (F) SolDM, t (5) = 14.44, ***p < 0.001; (F) SolDL, t (5) = 8.26, **p < 0.001; (F) PSol, t (5) = 7.58, **p < 0.01; (F) SolVL, t (5) = 15.52, ***p < 0.001 in multiple t-tests using the Holm-Sidak multiple comparison correction.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 5. Distribution of mCherry-positive neurons in different subdivisions of the cNTS following cVNS and aVNS A. Representative images showing subdivisions of the cNTS at Bregma -7.4 after cVNS (upper) or aVNS (lower). Scale, 100 μm. AP, area postrema; cc, central canal; SolDM, solitary nucleus dorsomedial part; SolDL, solitary nucleus dorsolateral part; SolM, solitary nucleus medial part; SolCe, solitary nucleus central part; Psol, parasolitary nucleus; SolVL, solitary nucleus ventrolateral part; ST, solitary tract; DMV, dorsal motor nucleus of the vagus. B. The number of mCherry+ neurons in different subdivisions in the cNTS from -7.24 to -7.79 to Bregma. (M + F) SolDM, t (13) = 17.86, ***p < 0.001; (M + F) SolDL, t (13) = 6.45, ***p < 0.001; (M + F) PSol, t (13) = 8.88, ***p < 0.001; (M + F) SolVL, t (13) = 14.60, ***p < 0.001; (M + F) SolC, t (13) = 3.60, **p < 0.01 in multiple t-tests using the Holm-Sidak multiple comparison correction. C-D. Sex disaggregated analysis. N = 4 in cVNS and 4 aVNS in males (C); 3 in cVNS and 4 aVNS in females (D). (M) SolDM, t (6) = 12.89, ***p < 0.001; (M) SolDL, t (6) = 3.90, *p < 0.05; (M) PSol, t (6) = 5.12, *p < 0.05; (M) SolVL, t (6) = 8.69, ***p < 0.001; (F) SolDM, t (5) = 14.44, ***p < 0.001; (F) SolDL, t (5) = 8.26, **p < 0.001; (F) PSol, t (5) = 7.58, **p < 0.01; (F) SolVL, t (5) = 15.52, ***p < 0.001 in multiple t-tests using the Holm-Sidak multiple comparison correction.

Techniques: Comparison

Fig. 6. Comparison of the sizes of mCherryþ neurons associated with cVNS or aVNS A-B. Representative images of mCherry+ neurons in different medial (A1-A4; B1-B4) and lateral (A5-A6; B5-B6) subnuclei of the cNTS associated with cVNS (A) or aVNS (B) in male mice. Scale, 10 μm. C-E. Sex-aggregated (C) and sex-disaggregated (D, male; E, female) comparisons of the neuronal area of mCherry+ neurons assessed at Bregma -7.4 mm. Horizontal lines in the violin plots represent the median. Dotted horizontal lines represent quartiles in each group. (M + F) cVNS medial vs lateral, Z = 7.78, ****p < 0.0001; (M + F) aVNS medial and lateral, Z = 8.00, ****p < 0.0001; (M) cVNS medial vs lateral, Z = 4.4, ****p < 0.0001; (M) aVNS medial and lateral, Z = 4.98, ****p < 0.0001; (F) cVNS medial vs lateral, Z = 6.74, ****p < 0.0001; (F) aVNS medial and lateral, Z = 6.13, ****p < 0.0001 in Dunn’s post-hoc test following the Kruskal-Wallis test. N is shown in the parentheses. The data were obtained from six mice (3M and 3F) mice in aVNS and cVNS.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 6. Comparison of the sizes of mCherryþ neurons associated with cVNS or aVNS A-B. Representative images of mCherry+ neurons in different medial (A1-A4; B1-B4) and lateral (A5-A6; B5-B6) subnuclei of the cNTS associated with cVNS (A) or aVNS (B) in male mice. Scale, 10 μm. C-E. Sex-aggregated (C) and sex-disaggregated (D, male; E, female) comparisons of the neuronal area of mCherry+ neurons assessed at Bregma -7.4 mm. Horizontal lines in the violin plots represent the median. Dotted horizontal lines represent quartiles in each group. (M + F) cVNS medial vs lateral, Z = 7.78, ****p < 0.0001; (M + F) aVNS medial and lateral, Z = 8.00, ****p < 0.0001; (M) cVNS medial vs lateral, Z = 4.4, ****p < 0.0001; (M) aVNS medial and lateral, Z = 4.98, ****p < 0.0001; (F) cVNS medial vs lateral, Z = 6.74, ****p < 0.0001; (F) aVNS medial and lateral, Z = 6.13, ****p < 0.0001 in Dunn’s post-hoc test following the Kruskal-Wallis test. N is shown in the parentheses. The data were obtained from six mice (3M and 3F) mice in aVNS and cVNS.

Techniques: Comparison

Fig. 7. Anterogradely labeled mCherryþ cNTS nerve terminals in the hindbrain areas mCherry labeled cNTS terminals in RVM (A), Vc and MRt (B), KF and elPB (C), MPB and LC (D), and LPB (E) in mice with cVNS. A′ to E′ represents images from mice with aVNS. A″ to E″ shows the magnified images of the insets in A′ to E.’ Scale, 100 μm (A to E and A′ to E′) or 50 μm (A″ to E″). Bregma location for RVM, Vc and MRt was -7.68 mm and for KF, elPB, MPB, LC and LPB it was -5.41 mm for both cVNS and aVNS. White are Nissl + neurons. PCRt, parvicellular reticular formation; RVM, rostral ventromedial medulla; IOC, inferior olive subnucleus C of the medulla; py, pyramidal tract; Sp5, spinal trigeminal tract; Vc, trigeminal spinal caudal nucleus; MRt, medullary reticular formation; elPB, externo-lateral part of the parabrachial nucleus; KF, K¨olliker-Fuse nucleus; SCP, superior cerebellar peduncle; Me5, mesencephalic trigeminal nucleus; LC, locus coeruleus; LPB, lateral parabrachial nucleus. F-G. Measurements of mCherry+ nerve terminal density in RVM (F) and LPB (G). N = 7 in cVNS and 8 in aVNS. Not significant in Student’s t-test.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 7. Anterogradely labeled mCherryþ cNTS nerve terminals in the hindbrain areas mCherry labeled cNTS terminals in RVM (A), Vc and MRt (B), KF and elPB (C), MPB and LC (D), and LPB (E) in mice with cVNS. A′ to E′ represents images from mice with aVNS. A″ to E″ shows the magnified images of the insets in A′ to E.’ Scale, 100 μm (A to E and A′ to E′) or 50 μm (A″ to E″). Bregma location for RVM, Vc and MRt was -7.68 mm and for KF, elPB, MPB, LC and LPB it was -5.41 mm for both cVNS and aVNS. White are Nissl + neurons. PCRt, parvicellular reticular formation; RVM, rostral ventromedial medulla; IOC, inferior olive subnucleus C of the medulla; py, pyramidal tract; Sp5, spinal trigeminal tract; Vc, trigeminal spinal caudal nucleus; MRt, medullary reticular formation; elPB, externo-lateral part of the parabrachial nucleus; KF, K¨olliker-Fuse nucleus; SCP, superior cerebellar peduncle; Me5, mesencephalic trigeminal nucleus; LC, locus coeruleus; LPB, lateral parabrachial nucleus. F-G. Measurements of mCherry+ nerve terminal density in RVM (F) and LPB (G). N = 7 in cVNS and 8 in aVNS. Not significant in Student’s t-test.

Techniques: Labeling

Fig. 8. Anterogradely labeled mCherryþ cNTS nerve terminals in periaqueductal gray (PAG) mCherry labeled cNTS terminals in the PAG of mice with cVNS (A) or aVNS (B). C shows a magnified image of the inset in B. Bregma level was registered at -4.83 mm. Scale, 100 μm in A-B and 50 μm in C. White are Nissl + neurons. DMPAG; dorsomedial periaqueductal gray; DLPAG, dorsolateral periaqueductal gray; VLPAG, ventrolateral periaqueductal gray; DRL, dorsal raphe nucleus lateral part, DRD, dorsal raphe nucleus dorsal part. D. Measurements of mCherry+ nerve terminal density in DLPAG. N = 7 in cVNS and 8 in aVNS. t (13) = 2.62, *p < 0.05 in Student’s t-test.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 8. Anterogradely labeled mCherryþ cNTS nerve terminals in periaqueductal gray (PAG) mCherry labeled cNTS terminals in the PAG of mice with cVNS (A) or aVNS (B). C shows a magnified image of the inset in B. Bregma level was registered at -4.83 mm. Scale, 100 μm in A-B and 50 μm in C. White are Nissl + neurons. DMPAG; dorsomedial periaqueductal gray; DLPAG, dorsolateral periaqueductal gray; VLPAG, ventrolateral periaqueductal gray; DRL, dorsal raphe nucleus lateral part, DRD, dorsal raphe nucleus dorsal part. D. Measurements of mCherry+ nerve terminal density in DLPAG. N = 7 in cVNS and 8 in aVNS. t (13) = 2.62, *p < 0.05 in Student’s t-test.

Techniques: Labeling

Fig. 9. Anterogradely labeled mCherryþ cNTS nerve terminals in the forebrain areas mCherry+ nerve terminals in the thalamic nuclei (A-B), amygdala (C), and hypothalamus (D) in mice with cVNS. A1 to D1 represents images from mice with aVNS. A2 to D2 show magnified images of the insets in A1 to D1. B3 shows the magnified image designated by the blue box in B1. Scale, 100 μm (A to D and A1 to D1) or 50 μm (A2 to D2). Bregma level for the thalamic nuclei was registered at -1.79 mm for cVNS and -1.91 mm for aVNS, respectively, and for the amygdala and hypothalamus, it was -1.43 mm for both the cVNS and aVNS. White are Nissl + neurons; Rt, reticular nucleus; VPL, ventral posterolateral nucleus; VPM, ventral posteromedial nucleus; VPPC, parvicellular part of the VPM; VM, ventral motor nucleus; ZID, zona incerta dorsal part, ZIV, zona incerta ventral part; ic, internal capsule; 3V, third ventricle; Hb, habenular nucleus; PV, periventricular thalamic nucleus; MDL, mediodorsal thalamic nucleus lateral part; MDC, mediodorsal thalamic nucleus central part; MDM, mediodorsal thalamic nucleus medial part; CM, centromedial thalamic nucleus; PC, paracentral thalamic nucleus; BLA, basolateral amygdala; CeC, central amygdala central part; CeL, central amygdala lateral part; CeM, central amygdala medial part; LH, lateral hypothalamus; f, fornix; DMH, dorsomedial hypothalamic nucleus; VMH, ventromedial hypothalamic nucleus; MTu, medial tuberal nucleus. E-G. Measurements of mCherry+ nerve terminal density in PV (E), central nucleus of the amygdala (F), and LH (G). N = 7 in cVNS and 8 in aVNS. Not significant in Student’s t-test.

Journal: Brain stimulation

Article Title: Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice.

doi: 10.1016/j.brs.2024.08.007

Figure Lengend Snippet: Fig. 9. Anterogradely labeled mCherryþ cNTS nerve terminals in the forebrain areas mCherry+ nerve terminals in the thalamic nuclei (A-B), amygdala (C), and hypothalamus (D) in mice with cVNS. A1 to D1 represents images from mice with aVNS. A2 to D2 show magnified images of the insets in A1 to D1. B3 shows the magnified image designated by the blue box in B1. Scale, 100 μm (A to D and A1 to D1) or 50 μm (A2 to D2). Bregma level for the thalamic nuclei was registered at -1.79 mm for cVNS and -1.91 mm for aVNS, respectively, and for the amygdala and hypothalamus, it was -1.43 mm for both the cVNS and aVNS. White are Nissl + neurons; Rt, reticular nucleus; VPL, ventral posterolateral nucleus; VPM, ventral posteromedial nucleus; VPPC, parvicellular part of the VPM; VM, ventral motor nucleus; ZID, zona incerta dorsal part, ZIV, zona incerta ventral part; ic, internal capsule; 3V, third ventricle; Hb, habenular nucleus; PV, periventricular thalamic nucleus; MDL, mediodorsal thalamic nucleus lateral part; MDC, mediodorsal thalamic nucleus central part; MDM, mediodorsal thalamic nucleus medial part; CM, centromedial thalamic nucleus; PC, paracentral thalamic nucleus; BLA, basolateral amygdala; CeC, central amygdala central part; CeL, central amygdala lateral part; CeM, central amygdala medial part; LH, lateral hypothalamus; f, fornix; DMH, dorsomedial hypothalamic nucleus; VMH, ventromedial hypothalamic nucleus; MTu, medial tuberal nucleus. E-G. Measurements of mCherry+ nerve terminal density in PV (E), central nucleus of the amygdala (F), and LH (G). N = 7 in cVNS and 8 in aVNS. Not significant in Student’s t-test.

Techniques: Labeling

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1) Product Images from "Acute stress and multicellular development alter the solubility of the Dictyostelium Sup35 ortholog ERF3"

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